火龙果果肉天然红色素的提取方法和条件-仲恺农业工程学院学报.PDF

仲恺农业技术学院学报, 19(4):17～21, 2006 Journal of Zhongkai University of Agriculture and Technology :1006-0774(2006)04-0017-05 1 2 ＊ , (1.仲恺农业技术学院设备处, 2.仲恺农业技术学院生命科学学院, 广东 广州510225) :., , 538 nm ; ;:pH 6.5、 V()∶m ()=8 ∶1、 40℃ 60 min. :;; :TS202.1　　　　　:A Study on the extraction of pigment from Hy locereus undutus flesh HANG Wei-feng , HE Sheng-gen (1.Division of Equipment, hongkai University of Agriculture and Technology, Guangzhou 510225, China; 2.College of Life Sciences, hongkai University of Agriculture and Technology, Guangzhou 510225, China) Abstract :The extraction conditions of a natural red pigment from flesh of Hy locereus undutus var.Taiwan Xi- anglong were studied.The results showed that the pigment was well water-and ethanol-soluble, and itsmaxi- mum absorption peakswavelength was at 538 nm.It was found that its extraction was significantly affected by different solvents.The best extraction conditionswere that the pH was 6.5, V(pure ethanol)∶m (flesh)=8 ∶1 and at 40 ℃for 60 min. Key words:Hy locereus undutus;natural red pigment;extraction 　　, 、 , , , [1-3] . (Hylocereus undutus )、 、、 、 , .、 、、, . [3-5] , ., , 、 , ., [5-11] , . , . 1　 1.1　 , . 　 :2006-09-04 : (C1020603). : (1964-), , , , .　＊:E-mail:heshenggen@ 18 19 1.2　 1.2.1　不同提取剂与果肉色素溶液呈色 关系　 2 g , 、 0.2 mol/L HCl、0.2 mol/L NaOH、 φ()=99.5%、φ()=99%、 φ()=99.5% 20 mL, 30 min, 8 000 r/min 20 min, 5 mL , 1.0 mol/L HCl NaOH pH 6.5, 50 mL, , 538 nm A538nm., φ()=20 %、40%、60%、80%100% φ()=20%、 40%、 60%、80%100%A538nm, ;0.05mol/L、 0.1mol/L、0.15 mol/L、0.2 mol/L 0.25 mol/L 、A538nm, . 1.2.2　不同提取剂对果肉色素 吸收光谱 影响　1.2.1 φ()