黄嘌呤氧化酶催化氧化降解β.pdf

烟草科技/ Tobacco Science Technology 烟草化学/ Tobacco Chemistry 2014 年第 10 期（总第327 期） 黄嘌呤氧化酶催化氧化降解ββ-胡萝卜素 刘 珊，胡 军，胡有持，宗永立，曾世通* 中国烟草总公司郑州烟草研究院，郑州高新技术产业开发区枫杨街2 号 450001 摘要：为开发新型烟用香原料，建立了一种酶催化氧化降解β- 胡萝卜素以产生香气物质的方法，并对黄 嘌呤氧化酶催化氧化降解β- 胡萝卜素的条件进行了研究，分别考察了反应相、酶用量及有机溶剂对产物 中香气物质生成量的影响。结果表明：①黄嘌呤氧化酶催化氧化降解β- 胡萝卜素时，能够生成异佛尔 酮、β- 环柠檬醛、β- 紫罗兰酮、二氢猕猴桃内酯等产物，这些化合物是烟草及烟草制品中的重要香气物 质；②与有机相体系和水相体系相比，水包油型两相体系更有利于香气物质的生成；③随着黄嘌呤氧化 酶用量的增加，产物中香气物质的生成量呈先增加后降低的趋势；④在两相体系中加入适量的低极性有 机溶剂能够提高黄嘌呤氧化酶的催化活力，同时避免中间产物的继续降解，有利于香气物质的保持。 关键词：β- 胡萝卜素；黄嘌呤氧化酶；氧化降解；烟草；香气物质 + 中图分类号：TQ656 .5 文献标识码：A 文章编号：1002-0861 （2014 ）10-0052-04 Oxidative Degradation of β-Carotene Catalyzed by Xanthine Oxidase LIU Shan, HU Jun, HU Youchi, ZONG Yongli, and ZENG Shitong* Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China Abstract: In order to develop a new tobacco flavor, a method of producing aromatic substance via enzyme catalysis of β-carotene was established. The conditions of oxidative degradation of β-carotene catalyzed by xanthine oxidase were studied, and the effects of reaction phases, enzyme amounts and organic solvents on the yield of aromatic substance were investigated. The results showed that: 1）The degradation products included isophorone, β-cyclocitral, β-ionone and dihydroactinidiolide, which were major aromatic substance in tobacco and tobacco products. 2 ）Comparing with the system of organic or water phase, the biphase system of oil-in-water was more beneficial to the formation of aromatic substance. 3）The yield of aromatic substance in the product increased at first and then decreased with the increase of xanthine oxidase. 4 ）Appropriate addition of low polar solvents to the oil-in-water system would improve the catalytic activity of xanthine oxidase and inhibit further degradation of intermediates. Keywords: β-Carotene; Xanthine oxidase; Oxidative degradat