Estimationofalternativesplicingisoformfrequenciesfrom培训课件.pptxVIP

Estimation of alternative splicing isoform frequencies from RNA-Seq data;Introduction EM Algorithm Results Conclusions and future work;RNA-Seq;Read ambiguity (multireads) What is the gene length?;Ignore multireads [Mortazavi et al. 08] Fractionally allocate multireads based on unique read estimates [Pasaniuc et al. 10] EM algorithm for solving ambiguities Gene length: sum of lengths of exons that appear in at least one isoform ? Underestimate expression levels for genes with 2 or more isoforms [Trapnell et al. 10] ;Read Ambiguity in IE;[JiangWong 09] Poisson model, single reads only [Li et al.