杆状病毒表达系统（昆虫细胞的培养及杆状病毒滴度测定）.docVIP

PAGE PAGE 2 杆状病毒表达系统（昆虫细胞的培养及杆状病毒滴度测定） 1．昆虫细胞的培养： Insect Cell Lines： Sf9 or Sf21 cells——主要用于转染，空斑纯化和滴度测定 High Five? cells or Mimic? Sf9 cells——转染效率低，主要用于蛋白表达 Graces Insect Medium with L-glutamine and supplemented with（pH 6.5）： 3330 mg/L lactalbumin hydrolysate 3330 mg/L yeastolate 350 mg/L NaHCO310% heat-inactivated fetal bovine serum Atmosphere：air, 100% Temperature：28.0 Growth conditions： The recommended media are formulated for use without CO2. Omission ofthe yeastolate or lactalbumin hydrolysate will lead to poor performance by this line. Subculturing：Gently resuspen