Exosomes Derived from Irradiated Esophageal Carcinoma-Infiltrating T Cells Promote Metastasis by Inducing the Epithelial–Mesenchymal Transition in Esophageal Cancer Cells 照射后食管癌浸润性T细胞的外体通过 导食管癌细胞的上皮-间充质转化促进转移.pdfVIP

Pathol. Oncol. Res. DOI 10.1007/s12253-016-0185-z ORIGINAL ARTICLE Exosomes Derived from Irradiated Esophageal Carcinoma-Infiltrating T Cells Promote Metastasis by Inducing the Epithelial–Mesenchymal Transition in Esophageal Cancer Cells Hua Min 1 Xiangdong Sun 2 Xi Yang 1 Hongcheng Zhu 1 Jia Liu 1 Yuandong Wang 1 Guangzong Chen 1 Xinchen Sun 1 Received: 20 September 2016 /Accepted: 29 December 2016 # Arányi Lajos Foundation 2017 Abstract Exosomes are nanovesicles derived from tumor through the upregulation of β-catenin and the NF-κB/snail and normal cells that are detectable in human biological fluids, pathway. Moreover, this study supports the use of exosomes such as plasma, and cell culture supernatants. The function of as a nearly perfect example of biomimetic nanovesicles that exosome secretion from Bnormal^ cells is unclear. Although could be utilized in future therapeutic strategies against vari- numerous studies have investigated exosomes derived from ous diseases, including cancer. hematopoietic cells, little is known regarding exosomes fromT cells, even though these cells play significant roles in Keywords Tcells . Radiotherapy . Exosome . Esophageal innate and acquired immunity. A CCK-8 assay was used to carcinoma . Epithelial–mesenchymal transition . Metastasis examine the ability of exosomes to inhibit TE13 cell prolifer- ation. In vitro invasion and wound healing assays were con- ducted to explore the effects of exosomes on TE13 cell migra- Introduction tion and invasion. A Western blottinganalys is was performed to investigate the effects of exosomes on the expression of the Esophageal cancer (EC) is the sixth most common cause of EMT-related molecules β-catenin, NF- κB and snail. This cancer-related deaths world wide, and squamous cell carcino- study aimed to investigate the eff