6 第六章 植物抗病基因工程的研究进展√.pptxVIP

第七章 植物抗病基因工程的研究进展;从三方面学习讨论;第一节 抗病基因工程的基本原理 The basic principles of disease resistance gene engineering;植物抗病基因工程主要包括内容:;Plant genetic engineering mainly includes:;第二节 已克隆到的基因;第三节 植物抗病基因工程策略与例子 ;一 正面策略：设法使目标基因表达 Front strategy: trying to make the target gene expression;(一) 抗病毒病基因工程 (A) Anti-Virus Genetic Engineering;1 外壳蛋白(CP )基因;Coat protein (coat protein) mediated resistance is the first study, but also far more successful anti-virus tools. For many viruses have so far been successfully constructed a variety of anti-viral plant, such as: tobacco mosaic virus (TMV), potato virus X (PVX), potato virus Y (PVY), alfalfa mosaic virus (ALMV), Floral mosaic virus (CMV) and other resistant plants.Current coat protein-mediated resistance mechanism is not very clear, it was believed that the viral coat protein (coat protein, CP) gene expression was transferred to the plant, due to the accumulation of coat protein inhibits virus infection early strip Shells so that the virus can not expose the internal nucleic acid particles, thereby preventing the viral replication and proliferation ;2 病毒复制酶基因;Viral replication gene;3 病毒卫星RNA, virus satellite RNA;Application of certain plant viruses with RNA virus satellite RNA satellite, the satellite RNA virus with altered virulence accompanied capabilities.It has now produced some carrying the virus satellite RNA of transgenic plants, such as transgenic tobacco satellite sequences and TRV CMV, and CMV satellite sequences reduce their associated virus - symptoms TAV (TAV) caused.However, the satellite RNA policy are still many problems:;1) 只有少数植物病毒具有卫星RNA; 2) 卫星RNA有可能被其自然的病毒载　　体包装并释放到环境中；3) 有些卫星RNA实际上能够增强病毒　　的致病力; 4) 存在潜在危险:一个核苷酸的突变可能造成严重病状，病毒卫星RNA可能和植物卫星RNA重组产生一种难以预料的病症，但目前尚未有良性卫星RNA突变成致病性很强的卫星RNA的报道。 ;1) Only a few plant viruses have satellite RNA;2) satellite RNA may be their natural viral vector packaged and released into the environment?;3) Some satellite RNA can actually enhance the virulence of the virus?;4) t