第25章心血管系统的发生.ppt

知识拓展：动态的血管发育（斑马鱼）心脏收缩引起的血流动力学作用 参考文献：Baldessari D1, Mione M. How to create the vascular tree? (Latest) help from the zebrafish. Pharmacol Ther. 2008 May;118(2):206-30. /science/article/pii/S0163725808000387 * * A) Intersegmental vessels formation: the scheme depicts one side of the embryo. Primary sprouts emerge bilaterally exclusively from the dorsal aorta (DA, red) and grow dorsally, where branches interconnect on either side of the trunk to form two dorsal longitudinal anastomotic vessels (DLAV)(背侧纵向吻合管). Secondary sprouts begin to emerge exclusively from the posterior cardinal vein (PCV, blue). Some secondary sprouts connect to the base of primary segments, becoming intersegmental veins (blue), while others do not and contribute to the parachordal vessels (pv). Primary segments that remain connected only to the dorsal aorta become intersegmental arteries (red). Intersegmental veins form additional connections to the parachordal vessels at the level of the horizontal myoseptum. B) Vessel lumen formation. The diagram depicts the sequence of events: intracellular vesicle formation, intracellular vesicle fusion, intercellular merging of vacuolar compartments and lumen formation. C) Model of the zebrafish intersegmental vessel (ISV). An ISV is composed of three types of endothelial cells. The dorsal connection to the DLAV is a T-shaped cell (blue); the ventral connection to the aorta is an inverted ‘T' (red). The connecting cell (green) courses between the somites ventral to the notochord-neural tube interface, and appears not to follow the somite boundary dorsally. DA, dorsal aorta; PCV, posterior cardinal vein; ISV, intersegmental vessel, DLAV, dorsal longitudinal anastomotic vessel, pc, parachordal vessel; S, somite. * Knock down of Sirt1 by morpholino injection showed that Sirt1 is required for proper vascular growth and maturation (Potente et al., 2007). Confocal images showing the GFP signal of Tg(fli1:eGFP)y1 embryos. Left: control embryo at 24 hpf, right: s