Genome Res.-1994-Edwards-S65-75英文文献资料.pdfVIP

Downloaded from on November 17, 2016 - Published by Cold Spring Harbor Laboratory Press ll|llllManual Supplement Multiplex PCR: Ever since it was shown that PCR could simultaneously amplify multiple loci in the human dystrophin gene, (1) multiplex PCR has been firmly established Advantages, as a general technique. A short list of multiplex PCR applications now in- Development, cludes pathogen identification, gender screening, linkage analysis, forensic studies, template quantitation, and genetic disease diagnosis. Multiplex PCR and can be a two-amplicon system or it can amplify 13 or more separate regions Applications of DNA. It may be the end point of analysis, or preliminary to further analyses such as sequencing or hybridization. The steps for developing a multiplex PCR and the benefits of having multiple fragments amplified simultaneously, however, are similar in each system. This review focuses on multiplex systems in which each primer pair targets Mary C. Edwards and a single locus, unlike RAPD(2) and alumorph {3)PCR reactions, which amplify Richard A. Gibbs multiple loci with a single primer or primer set. Institute for