新型试管花卉姬松高效再生体系的研究-园艺学报.pdf

园 艺 学 报 2013 ，40 （11）：2287 –2294 http: // www. ahs. ac. cn Acta Horticulturae Sinica E-mail: yuanyixuebao@126.com 新型试管花卉姬松高效再生体系的研究 * 康冬茹，郭先锋 ，李圆圆，胡小蓉 （山东农业大学林学院，山东泰安 271018 ） 摘 要：以新型试管花卉多肉类植物姬松（Crassula clavata N. E. Brown ）为材料进行高效再生体系 的研究。结果表明：以姬松茎尖为外植体的初代培养中，MS 培养基添加 IBA 并未诱导出根系生成，而是 直接诱导出丛生芽，在 MS + IBA 0.3 mg · L-1 培养基上，由叶片表面诱导出的丛生芽质量最佳；在继代培 养中，最佳培养基为 MS + NAA 0.1 mg · L-1 + 6-BA 0.5 mg · L-1 ，诱导产生大量胚性愈伤组织，且表面有 芽体分化；在彩色培养中，在 1/2MS 中添加0.25 mg · L-1 的食用色素亮蓝，观赏效果佳且对植株生长无毒 害。 关键词：姬松；快繁；试管花卉；再生体系 中图分类号：S 68 文献标志码：A 文章编号：0513-353X （2013 ）11-2287-08 A Highly Efficient in Vitro Regeneration Protocol for Crassula clavata as Novel Tube-flower * KANG Dong-ru ，GUO Xian-feng ，LI Yuan-yuan ，and HU Xiao-rong （Colloge of Forestry，Shandong Agricultural University ，Tai’an，Shandong 271018 ，China ） Abstract ：Tube-flower is a novel form of in vitro plant products. A highly efficient in vitro regeneration system was established for the production of succulent plant Crassula clavata N. E. Brown. In primary culture ，adventitious buds instead of adventitious roots were differentiated from the shoot explants on MS medium supplemented with different concentrations of IBA ；The highest multiplication rate and high-quality adventitious buds were obtained on the optimal medium of MS containing 0.3 -1 -1 -1 mg · L IBA. In subculture ，the medium of MS containing 0.1 mg · L NAA and 0.5 mg · L 6-BA was screened out for large amounts of embryogenic callus could be significantly induced and sprouts could be