2013南方科技大学课程考核试卷A卷普通生物学mostupdatedanswer.docVIP

Name: _______ Class: _______ Date: _______ Student ID: _______ GENERAL BIOLOGY FINAL EXAM (A) Question 1: Multiple-Choice Questions (Chose one answer only, 60%, 2% each) C 1) Certain nutrients are considered essential in the diets of some animals because A) only those animals use those nutrients. B) the nutrients are subunits of important polymers. C) these animals are not able to synthesize these nutrients. D) the nutrients are necessary coenzymes. E) only certain foods contain them. E 2) A vitamin that is especially important for preventing neural tube abnormalities in a fetus is A) vitamin A B) vitamin C C) vitamin K D) vitamin B2 E) folic acid（叶酸） C 3) What is the most logical sequence of steps（合理的顺序） for splicing（剪切）foreign DNA into a plasmid（质粒）and inserting the plasmid into a bacterium? I. Transform bacteria（转化细菌）with a recombinant DNA molecule. II. Cut the plasmid DNA using restriction enzymes（限制性内切酶）. III. Extract plasmid DNA from bacterial cells. IV. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments. V. Use ligase to seal plasmid DNA to nonplasmid DNA. A) I, II, IV, III, V B) II, III, V, IV, I C) III, II, IV, V, I D) III, IV, V, I, II E) IV, V, I, II, III A 4) A researcher has used in vitro mutagenesis（体外突变）to mutate a cloned gene and then has reinserted（重新插入）this into a cell. In order to have the mutated sequence disable the function of the gene, what must then occur? A) recombination（重组）resulting in replacement of the wild type with the mutated gene B) use of a microarray（基因芯片）to verify continued expression of the original gene C) replication（复制）of the cloned gene using a bacterial plasmid D) transcription（转录）of the cloned gene using a BAC E) attachment of the mutated gene to an existing mRNA to be translated B 5) Most ____ tissues are dominated by the extracellular matrix. A) epithelial B) connective C) nerve D) muscle E) smooth muscle D 6) Which of the following can form an almost impermeable seal（防渗密封带） between two adjacent epithelial cell