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Government of Canada Gouvernement du Canada
Laboratory Procedure MFLP-42
May 2011
Health Products and Food Branch
Ottawa
Isolation and enumeration of the Bacillus cereus group in foods
1 2 3
Donna Douey , Irene Iugovaz , and Rick Szabo
1Calgary Laboratory, Canadian Food Inspection Agency, 3650 36th St. N.W., Calgary, AB, T2L 2L1
2
Food Microbiology, Health Products and Food Branch (HPFB), Health Canada, 1001, St-Laurent ouest,
Longueuil, Québec, J4K 1C7
3
Microbiology Evaluation Division, Bureau of Microbial Hazards, Health Products and Food Branch,
Health Canada, Postal Locator 2204E, Ottawa, Ontario, K1A 0L2
E-mail: micro_methods_committee@hc-sc.gc.ca
1. Application
This method is applicable to the isolation, identification and enumeration of the Bacillus cereus group (with
limitations as described in the method) in foods in accordance with the requirements of Sections 4 and 7 of the
Food and Drugs Act. The revised method replaces MFLP-42, dated April, 2003.
2. Description
The method has been shown to produce satisfactory results with naturally-contaminated meats, vegetables,
dairy products, cereals and dried foods (8.5).
3. Principle
The Bacillus cereus group is widely distributed in nature, consisting of B. c
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