原位移植法建立肝脏h22肝癌细胞移植瘤小鼠模型.pdfVIP

World Journal of Cancer Research 世界肿瘤研究, 2015, 5, 15-20 Published Online January 2015 in Hans. /journal/wjcr /10.12677/wjcr.2015.51003 Orthotropic Transplantation to Establish H22 Hepatocellular Carcinoma Model in Mice Chen Han1,2, Hengxiao Wang1,2*, Zhaoxia Wang3 1 Department of Immunity, Institute of Basic Medicine, Shandong Academy of Medical Sciences, Ji’nan 2 School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Ji’nan 3 Department of Pathology, Institute of Basic Medicine, Shandong Academy of Medical Sciences, Ji’nan * Email: wanghengxiao@ th nd th Received: Nov. 24 , 2014; revised: Dec. 22 , 2014; accepted: Dec. 30 , 2014 Copyright © 2015 by authors and Hans Publishers Inc. This work is licensed under the Creative Commons Attribution International License (CC BY). /licenses/by/4.0/ Abstract Objective: To establish a liver orthotropic transplantation tumor model with H22 cells, for the further development of the related experimental study. Methods: A certain number of H22 cells were directly injected in the left lobe of the liver in C57BL/6 mice, establishing a transplanted tu- mor model of hepatocellular carcinoma in situ. Results: After 7 days of surgery, the liver surface appeared irregular size nodules, nodules increased with the prolongation of time increases, the late abdominal organs appeared invasion, adhesion, ascites was increased. Histopathological ex- amination of liver tumor tissues and adjacent tissues of cancer showed that liver orthotropic transplantation tumor formation rate was 100%, no spontaneous reg