分流不分流进样口介绍.ppt

分流进样的实用性 分流进样适合于大部分可挥发样品，包括液体和气体样品，特别是对一些化学试剂的分折。因为其中一些组分会在主峰前流出。而且样品不能稀释、故分流进样住往是理想的选择。此外，在毛细管GC的方法开发过程中，如果对样品的组成不很清楚。也应首先采用分流进样口。对于一些相对“脏”的样品，更应采用分流进样，因为分流进样时大部分样品被放空，只有一小部分样品进入色谱柱，这在很大程度上防止了柱污染。 Slide #* Slide #* Septum SEPTUM PURGE VENT Inlet Split Port Slide #* Slide #* Slide #* Slide #* Slide #* Slide #* Slide #* Slide #* Slide #* Slide #* Slide #* Slide #* * Split Splitless Inlet Component Identification: The drawing on the left will be used to demonstrate Split operation of the Agilent 6850 and 6890 Split/Splitless Capillary Inlet. The internal components in the injection port are as follows: 1) Septum 2) Inlet Liner 3) Liner O-Ring seal 4) Gold Inlet seal 5) Capillary Column * The Capillary Inlet is shown in Split Mode with no flow or pressure setpoints entered. * The Inlet Total Flow is set to 205 ml/minute. Proportional valve 1 opens to introduce flow, and then controls at the setpoint. The flow path is through the inlet liner, around the outside of the liner and out the split vent through the filter, Purge Valve and Proportional Valve 2. Proportional valve 2 is completely open until a pressure setpoint is entered. * An Inlet pressure of 10 PSI is entered. 10 PSI is the pressure required to establish 2 ml/minute of flow on this particular column at the oven starting temperature. Proportional valve 2 starts to close off, increasing Inlet back pressure, then controlling at 10 PSI. Once the inlet pressurizes, the septum purge and column flows are established. The Total flow is equal to the sum of Column + Split Vent + Septum Purge flows. * The Capillary Inlet is Ready for Injection. The split ratio is equal to the split vent flow rate divided by the column flow rate - in this example 100:1. * The sample syringe is inserted into the injection port. * The plunger is depressed, injecting liquid sample into the inlet. * The sample is vaporized. It is important to be sure that the inlet liner has sufficient internal volume to contain the solvent vapor. The a