石榴品种资源的RAPD亲缘关系分析-河南农业科学.pdfVIP

DOI牶牨牥牣牨牭牴牫牫牤j牣c ki牣牨牥牥牬牠牫牪牰牳牣牪牥牥牱牣牥牪牣牥牪牨 河南农业科学 RAPD * 杨荣萍, 龙雯虹, 杨正安, 李文祥 ( , 650201) :以36 份石榴品种(类型)为研究对象,用 RAPD 技术对其亲缘关系进行分析。从128 个随机 引物 筛选出12 个重复性好、条带清晰的多态性引物, 并用这些引物对36 份材料进行 RA PD 扩 增, 共扩增出111 条谱带,平均每 个引物9. 25 条。12 个引物扩增出不同的多态性条带, 多态性程 度达到72. 07%。用Statistic 分析软件计算出的遗传距离在0. 027 ～0. 441 之间,并对36 份材料 进行了UPGA 法聚类, RAPD 结果的亲缘关系树状图显示, 36 个品种或类型基因背景较复杂, 说 明了我国的石榴栽培品种有丰富的种质资源。 :石榴;种质资源;RA PD;聚类分析;亲缘关系 :S665. 4 　　:A 　　:1004 3268(2007)02 0069 04 Phyloge etic Relatio ship amo g P un ica g rana tum L. Germplasm Resources Based o RAPD M arkers * YANG Ro g-pi g, LONG We-ho g, YANG Zhe g-a , LI We-xia g (College of La dscape a d Horticulture, Yu a Agricultural U iversity, Ku mi g 650201, Chi a) Abstract :36 materials of P un ica gr anat um L. w ere a alyzed usi g ra dom amplified polymorphic DNA markers i this paper. 12 primers which had good repeat a d clear ba ds w ere scree ed from 128 arbitrary 10-mer primers. 12 ra dom primers w ere used to amplify 36 pomegra ate samples. The total 111 ba ds w ere obtai ed a d the average ba ds per ra dom primer w ere 9. 25. These ba ds displayed polymorphism. The ge etic diversity degree w as 72. 07%. The ge etic dista ce of 36 materials w as betw ee 0. 027 a d 0. 441 by the accou t of statistic a alysis softw are. A tree diagram of phyloge etic relatio ship w as co structed usi g UPGA accordi g to these data. The results show ed that the ge etic backgrou d of P unica g rana tum L. germplasm resources w as too complex a d abu da t to be classified. Key words:P un ica g rana tum L. ;Germplasm resource;RAPD;Cluster a alysis;Phyloge etic relatio ship