β防御素3在耐甲氧西林金黄色葡萄球菌呼吸机相关性肺炎大鼠肺组织中的.docVIP

β-防御素-3在耐甲氧西林金黄色葡萄球菌呼吸机相关性肺炎大鼠肺组织中的表达 作者单位：430022 武汉，华中科技大学同济医学院附属协和医院麻醉科 华中科技大学同济医学院附属协和医院麻醉科，武汉 430022 武庆平 桂平 姚尚龙 【摘要】 目的 研究大鼠耐甲氧西林金黄色葡萄球菌（MRSA）呼吸机相关性肺炎（VAP）时肺组织中β-防御素-3（BD-3）的表达，以探讨其在VAP发病机制中的作用。方法 20只清洁级雄性SD大鼠随机分为2组（n=10），非通气肺炎组（N组）和呼吸机相关性肺炎组（V组）。N组大鼠经口气管插管后立即接种2×109菌落形成单位（cfu）的MRSA，V组大鼠经口气管插管并进行4h保护性机械通气后气管内接种相同数量的细菌。接种后48 h处死大鼠，测定肺湿重与体重比（WW/BW），记录肺肉眼和病理学评分，并进行肺组织细菌计数和脾培养，免疫组化检测肺组织BD-3的表达。结果 与N组比较，V组WW/BW，肺病理学积分，肺组织细菌含量和菌血症发生率均显著升高（P均0.01），而BD-3的表达显著降低（P0.01）。结论 耐甲氧西林金黄色葡萄球菌呼吸机相关性肺炎（MRSA VAP）显著重于非通气肺炎，这可能与机械通气降低了BD-3的表达有关。 【关键词】耐甲氧西林金黄色葡萄球菌；呼吸机相关性肺炎；防御素 Expression of beta-defensin-3 in lungs of rats with methicillin-resisitant Staphylococcus aureus ventilator-associated pneumonia GUI Ping Department of Anesthesiology , Union Hospital , Tongji Medical College , Huazhong University of Science and Technology , Wuhan 430022 , China [Abstract] Objective To study the expression of beta-defensin-3(BD-3) in lungs of rats with methicillin-resisitant Staphylococcus aureus(MRSA) ventilator-associated pneumonia(VAP) and to investigate the role of BD-3 in the pathogenesis of VAP. Twenty specific pathogen-free male Sprague-Dawley rats weighing 242～286g were randomly divided into 2 groups: nonventilated pneumonia group(group N, n=10) and ventilator-associated pneumonia group(group V, n=10). Rats in group N were infected intrabronchially with 2×109 colony-forming units(cfu) MRSA immediately after oral intubation while rats in group V were infected intrabronchially with the same amount of MRSA after oral intubation and 4 hours, protective mechanical ventilation. Rats from both groups were killed 48 h after inoculation. Lung wet weight to body weight ratios(WW/BW) were measured, lung macroscopic and microscopic scores were recorded, lung bacterial counts and spleen culture were also done. Expression of BD-3 in lung of rats was detected by immunohistochemistry staining. Results WW/BW, lung microscopic score, bacterial pulmonary concentration and the incidence of bac