《杨梅黄酮对酒精性肝损伤小鼠肝脏组织匀浆中酶活的影响》-毕业设计（论文）.docVIP

浙江万里学院毕业论文 PAGE 本科毕业论文(设计) 论文题目：杨梅黄酮对酒精性肝损伤小鼠肝脏组织匀浆中酶活的影响 所在学院 生物与环境学院 专业班级 食品质量与安全061 学生姓名 学号 指导教师 职称 教授 完成日期 2010 年 5 月 10 日 摘 要 采用酒精灌胃法制造急性小鼠肝损伤模型，并选用荸荠种杨梅黄酮提取物作为干预物，采用试剂盒以及生化分析等手段，探讨了杨梅黄酮对酒精性肝损伤小鼠肝匀浆酶活性的影响，结果表明：经乙醇诱导的急性肝损伤小鼠预先摄入杨梅黄酮中、高剂量后，其肝匀浆的ADH、GPX、GST、GSH、SOD、CAT活性水平较模型组显著提高（P0.01），低剂量组无统计学意义。高剂量组中MDA、NO、NOS的含量明显降低（P0.01），而低剂量组无统计学意义。本研究结果揭示杨梅黄酮物质对酒精性肝损伤具有一定的防治效果。 关键词：杨梅黄酮；酒精性肝损伤；肝匀浆；酶活性 ABSTRACT Administration by gavage with alcohol was applied to manufacture a acute hepatic injury model of mice, the flavonoid crude extracts from Waternut Chinesebayberry was adopted as intervention. By the methods of reagent box detection and biochemical analysis, the effect of acute liver injury of mice induced by alcohol and intervention of flavonoid crude extracts from Chinesebayberry on the enzymatic activity of liver homogenate in mice were studied. The result showed that in the mice treated with flavonoid crude extracts from Chinesebayberry in middle and high dosages , the activities of ADH、GPX、GST、GSH、SOD and CAT in the liver homogenate were significantly enhanced compared with model group（P0.01）; Meanwhile, the content of MDA、NO and NOS in the high-dose group were significantly decreased（P0.01）, whereas, not statistically significant in the low-dose group. The study revealed that flavonoid crude extracts from Chinesebayberry could prevent and cure alcoholic liver injury at some extent. Key words: Myricetin; Alcoholic liver injury; Liver homogenate; Enzymatic activity 浙江万里学院毕业论文 PAGE II 目 录 TOC \o 1-4 \h \z \u 1 前言 1 2 材料与方法 2 2.1 材料 2 2.1.1 植物材料 2 2.1.2 动物材料 2 2.1.3 主要化学药品和试剂 2 2.1.4 主要仪器和设备 3 2.2 方法 3 2.2.1 杨梅黄酮提取物的制备 3 2.2.2 小鼠急性酒精性肝损伤的制造和分组 3 2.2.3 组织样本的采集和处理 4 2.2.4 测定指标及方法 4 2.2.4.1杨梅黄酮提取物对小鼠一般生理状况的影响 4 2.2.4.2杨梅黄酮提取物对小鼠肝匀浆各指标的影响 4 2.2.5 测定方法 5 2.2.5.1小鼠肝匀浆GST、GSH、SOD、CAT活性的测定 5 2.2.5.2小鼠肝匀浆ADH、T-AOC、GSH-PX水平的测定 5 2.2.5.3小鼠肝匀浆MDA、NO、NOS水平的测定