利用FRET检测DNA杂化过程.docVIP

Detection with Blue Luminescent Quantum Dots and Dye-Labeled Single-Stranded DNA Hui Peng,Lijuan Zhang,Tanja H.M.Kja ¨llman,Christian Soeller,and Jadranka Travas-Sejdic* Polymer Electronic Research Centre,The Uni V ersity of Auckland,Pri V ate Bag 92019,Auckland,New Zealand Received November 28,2006;E-mail:j.travas-sejdic@auckand.ac.nz Quantum dots (QDshave become an important photonic tool in the past two decades due to their unique properties,such as high chemical stability,resistance to photodegradation,and readily tunable optical properties.1-3QD colloids are often prepared using organometallic routes at high temperature.4-6The most widely used surface-capping ligands are trioctylphosphine/trioctylphosphine oxide (TOP/TOPOand long-chain alkylamine.The resulting QDs are hydrophobic,and further chemical modifications are generally required for compatibility with biological applications.Since the first reports on the designs of hydrophilic QDs and QD -protein conjugates,1,2a number of surface functionalization schemes have been developed to make QDs water soluble and thus suitable for biological applications.3,7,8An alternative approach is to synthesize QDs directly in an aqueous medium.Since the original report on the aqueous synthesis of mercaptoethanol-and thioglycerol-capped CdTe QDs,9significant progress has been made in the preparation of thiol-capped CdTe QDs that exhibit very stable luminescence.10 Due to their tunable narrow-band emission and broad excitation spectra QDs are excellent donors for fluorescence resonance energy transfer (FRET-based biosensors.Several sensor designs based on FRET between QDs and dye-labeled biomolecules have been developed.11-13For example,Mauro et al.designed a maltose-binding assay based on FRET between CdSe/ZnS QDs and dye acceptors.11A FRET TNT sensor was developed based on a hybrid QDs antibody fragment,12and Gill et al.used FRET in CdSe/ZnS -DNA conjugates to probe hybridization and DNA cleavage.13More recently,QD and