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Cat. # 3340 For Research Use Chaperone Plasmid Set Product Manual v201405 Cat. #3340 Chaperone Plasmid Set v201405 Table of Contents I. Description 3 II. Components 3 III. Storage 5 IV. Protocol 5 V. FAQs 6 VI. References 8 VII. Related Products 9 Safety Precautions Because the araB promoter and araC gene derived from Salmonella typhimurium are present on the Chaperone Plasmids pG-KJE8, pGro7, pKJE7, and pTf16, please follow all relevant guidelines for experiments using recombinant DNA as indicted by your organization when using this prod- uct. 2 URL: Cat. #3340 Chaperone Plasmid Set v201405 I．Description Large-scale expression of recombinant proteins is essential for structural and func- tional analyses of proteins. A variety of protein expression systems have been devel- oped to produce high levels of protein. Escherichia coli is commonly used as a host for protein expression, since it is a simple system that can be used to express a wide variety of proteins. However, expression of protein in E. coli often results in various problems, such as the formation of inclusion bodies and protease degradation of the protein. These frequently encountered issues often are a result of im