小麦醇溶蛋白自组装纳米粒子负载白藜芦醇的性质研究.docxVIP

基金项目：国家级大学生创新创业训练计划（项目编号 201510295018） 收稿日期：2016-10-25 作者简介：孔祥珍，女，1980年出生，副教授，植物蛋白科学与技术 小麦醇溶蛋白自组装纳米粒子负载白藜芦醇的性质研究 孔祥珍 黄 飞 周治彤 吴炜豪 （江南大学食品学院；食品科学与技术国家重点实验室，无锡214122） 摘 要 小麦醇溶蛋白是谷朊粉的主要成分，用小麦醇溶蛋白包埋白藜芦醇以达到稳定白藜芦醇的目的。用反溶剂法制得其自组装纳米粒子，对乙醇浓度、蛋白浓度、分散液pH、分散液盐浓度等因素对纳米粒子的粒径以及Zeta电位的影响做出了探究。发现在乙醇浓度为65%，蛋白质浓度4%，分散液pH为4.5，分散液盐浓度为0.00 mol/L时，得到理想的纳米粒子，其粒径约为（185.1±8.5） nm ,Zeta电位为（19.36±1.03） mV。而后在最佳条件下用小麦醇溶蛋白纳米粒子负载白藜芦醇，发现该复合物在芯壁比为1︰40时包埋率最高，此时粒径（166.7±3.7） nm。 Zeta电位(15.68±0.74) mV，包埋率为55.0%，载药率为1.5%。小麦醇溶蛋白纳米粒子可以用来稳定白藜芦醇。 关键词 小麦醇溶蛋白 纳米粒子 自组装 白藜芦醇 中图分类号：TQ218 文献标识码：A 文章编号：1003-0174（ ） Characterization of Gliadin Self-Assembly Nanoparticles Loaded with Resveratrol Kong Xiangzhen Huang Fei Zhou Zhitong Wu Weihao ( State Key Laboratory of Food Science and Technology；School of Food Science and Technology, Jiangnan University, Wuxi 214122) Abstract Gliadin is an important fraction of wheat gluten. Resveratrol was buried in the gliadin nanoparticles which stabilize the resveratrol. In this paper, gliadin nanoparticles were self-assembled by anti-solvent method. Effects of ethanol concentration, protein concentration, pH and salt concentration of the dispersion solution on the particle size and Zeta potential of the prepared nanoparticles were systematically studied. The desired nanoparticles, of which the mean diameter was （185.1±8.5） nm, and the mean Zeta potential was( 19.36±1.03) mV, were prepared under the condition of 65% ethanol concentration, 4% protein concentration，pH 4.5 and 0.00 mol/L salt concentration. Under the optimum conditions, the gliadin nanoparticles loaded with resveratrol have best encapsulation efficiency when prepared with 1︰40 core/wall ratio. The result showed that the prepared nanoparticles have 55.0% encapsulation efficiency and 1.5% drug loading rate with mean diameter of （166.67±3.7） nm, and mean Zeta potential of （15.68±0.74） mV. Gliadin nanoparticles can stabilize resveratrol. Key words gliadin, nanoparticle, self-assembly, resverat