上皮-part2-公务员考试.docxVIP

上皮-part2-公务员考试 FigureS2.EMT-AssociatedAutocrineSignaling,RelatedtoFigure2 (A)RT-PCR:mRNAexpressionofBMPligandsinHMLE24+,HTwistandMSPcells,n=3. (B)Luciferasereporterassay:Smadtranscriptionalactivity:cellsweretransfectedwithSBE4-lucreporterplasmid, re yluciferaselevelswerenormalizedtopGL-SV40renillatransfectioncontrol;cellsweretreatedwithrecombinantTGF-b1(5ng/ml)for30min,n=3. Cell145,926C940,June10,***-*****ElsevierInc.S5 RByahcetoi (C)RT-PCR:mRNAexpressionofSFRPisoformsinHMLE24+andMSPcelllines,n=3. (D)RT-PCR:mRNAexpressionofWntligandsinHMLE24+,HTwistandMSPcelllines,n