神经生长因子诱导神经母细胞瘤分化的研究.doc

神经生长因子诱导神经母细胞瘤分化的研究 目录 TOC \o "1-9" \h \z \u 目录 1 正文 2 文1：神经生长因子诱导神经母细胞瘤分化的研究 2 1 资料与方法 3 2 结果 5 3 讨论 5 文2：广藿香愈伤组织诱导和分化再生植株的研究 7 1材料和方法 8 11材料试验 8 12方法 9 121外植体培养 9 122愈伤组织的诱导 9 123愈伤组织的分化 9 124无根苗的诱导 9 13培养条件 10 2结果与分析 10 21愈伤组织的诱导 10 211不同植物激素对愈伤组织诱导的影响 10 212不同浓度BA对愈伤组织诱导的影响 11 213不同外植体诱导愈伤组织能力的差异 11 22愈伤组织的增殖 12 221不同植物激素对愈伤组织增殖的影响 12 222愈伤组织培养时间对增殖的影响 12 23愈伤组织的分化 13 231不同植物激素对愈伤组织分化的影响 13 232不同外植体诱导的愈伤组织分化能力的差异 13 24无根苗诱导生根及移栽 14 参考文摘引言： 15 原创性声明（模板） 17 文章致谢（模板） 17 正文 神经生长因子诱导神经母细胞瘤分化的研究 文1：神经生长因子诱导神经母细胞瘤分化的研究 【Abstract】 Objective To investigate the differentiation and proliferation of neuroblastoma(NB) cells induced by nerve growth factor(NGF) and its possible mechanisms. Methods NB tumor tissues were collected and primary cell culture was performed. Trypan blue exclusion was used to count the alive cells. Morphological changes were observed under the phase-contrast microscope. The change of cell proliferation was determined by mea of MTT assay. TrkA expression was examined by RT-PCR method. Results By day 2, the alive cells of group A、B and C decreased by treatment of NGF; morphological changes of NB cells were also observed. RT-PCR detection showed that the TrkAmRNA expression increased from day 2 and reached its peak on day 8 in a time and dose dependent manner by treatment of NGF. Conclusion NB cells treated by NGF differentiate to neuron-like cells and the cell growth is inhibited in a dose-dependent manner. TrkA's expression is increased by NGF. This may be one of the mechanisms of spontaneous regression of NB. 【Key words】 neuroblastoma nerve growth factor cell differentiation 神经母细胞瘤(neuroblastoma，NB)是起源于胚胎期神经嵴细胞的儿童恶性肿瘤， 自然 退化常见于新生儿、婴儿和早期肿瘤，而较大儿童晚期患者恶性程度高，预后差。对化疗耐药是 治疗 失败的主要原因［1］。如何应用人为方法使NB细胞分化、逆转、消退，是当今肿瘤界研究的热点问题［2］，用药物诱导NB向良性转化可能成为临床治疗的一个新途径。 1 资料与方法 一般资料 5岁男性患儿，因发现“腹部肿物”1周，于2004年6月10日入住本院，行腹部B超、CT检查，考虑为神经源性肿瘤；于2004年6月12日行剖腹探查、腹部肿物切除术