圆二色光谱cd教学文稿.pptVIP

;1.什么是圆二色谱?;?;;;X;;X;X;频率相同,方向相反 振幅相同、相位不同;? ;? ; 合成光特性;椭圆偏振光;振动方程;圆二色性与振幅 ;;旋光色散与位相;平均残基旋光度 [m]l the mean residue rotation; Plane (linearly) polarized light;;Cotton effect;;Instrument;CD application;;克山病探究-硒缺乏;烟草花叶病毒侵染膜蛋白;;Unfolding/folding;Conformational varies;;; Near UV spectra: to investigate the tertiary structure;振动圆二色谱;Protein Concentration: 0.5 mg/ml Cell Path Length: 0.5 mm Stabilizers (Metal ions, etc.): minimum Buffer Concentration : 5 mM or as low as possible while maintaining protein stability;参考文献;P30页倒数13，14行漏印了一段，应改为： 偶极子置于离子所产生的静电场中时，二者间的相互作用称 为电荷-偶极相互作用。例如：K+离子和其外