浅析亚洲带绦虫膜联蛋白B2基因的表达纯化及免疫学药学（药学资料）.docVIP

浅析亚洲带绦虫膜联蛋白B2基因的表达纯化及免疫学药学（药学资料） 文档信息 主题： 关于“医学心理学”中“药学”的参考范文。 属性： F-01L4K5，doc格式，正文10644字。质优实惠，欢迎下载！ 适用： 作为医学资料、药学资料写作的参考文献，解决如何写好实用应用文、正确编写文案格式、内容摘取等相关工作。 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：浅析亚洲带绦虫膜联蛋白B2基因的表达纯化及免疫学药学 1 1材料与方法 2 文2：细粒棘球蚴重组HSP70基因的表达纯化及免疫学鉴定 6 1 材料与方法 7 2 结果 9 3 讨论 10 参考文摘引言： 11 原创性声明（模板） 12 文章致谢（模板） 12 正文 浅析亚洲带绦虫膜联蛋白B2基因的表达纯化及免疫学药学（药学资料） 文1：浅析亚洲带绦虫膜联蛋白B2基因的表达纯化及免疫学药学 ABSTRACT: ObjectiveTo clone and express the gene named as Annexi B2 of Taenia saginata asitica so as to analyze the immunogenicity of its recombinant online analysis of the gene at bioinfomatics websites to identify the gene from the Taenia saginata asiatica full-length cDNA plasmid library, the gene was cloned into prokaryotic expression vector pET-28a(+) and then traformed to BL21 with IPTG induction. Thereafter, the gene product was analyzed by SDSand purified by Ni-NTA affinity chromatography. In addition, the immunoreactivity of the purified recombinant protEI was analyzed by Western demotrated by PCR, double enzyme digestion and DNA sequencing indicated that the recombinant plasmid was successfully cotructed and highly expressed. The recombinant protein tested with Western blotting analysis could react with Taenia asiatica and Taeniarhynchus saginatus infected patient Annexi B2 of Taenia saginata asitica has been cloned and expressed in the prokaryotic expression system and the purified protein has been confirmed with immunogenicity. KEY WORDS: Taenia saginata asiatica; Annexi B2; gene cloning; immunoreactivity 鉴于亚洲带绦虫与猪带绦虫及牛带绦虫在起源、分类学地位存在的差异[1-2]，本课题组率先构建亚洲带绦虫成虫全长cDNA质粒文库，并对该虫进行功能基因组的研究工作[3]。本文利用生物信息学工具从文库中筛选到了一个膜联蛋白B2(Annexi B2)的同源基因，构建了原核表达载体，并对其原核表达产物进行免疫学方面的初步研究。 1材料与方法 材料 血清、载体与菌株3种人体带绦虫患者血清取自粪检阳性的带绦虫患者。原核表达质粒pET-28a(+)及大肠埃希菌BL-21/DE3由中山大学热带病防治教育部重点实验室惠赠。 主要试剂和工具酶Ex Taq酶(含dNTP)，BamHⅠ，XhoⅠ，T4 DNA连接酶 (大连宝生物工程公司);异丙硫代-β-D半乳糖苷(IPTG)(美国Promega公司);质粒纯化试剂盒(广州东盛科技公司);辣根过氧化物酶标记的山羊抗猪IgG二抗、兔抗人二抗、3，3二氨基联苯胺(DAB)显色试剂盒(武汉博士德生物工程有限公司);氯化钙、氯化钠等为国产分析纯试剂。