Agilent 10 x more sensitivity with the Agilent 1290 1260 Infinity Diode Array Detector compared to Agilent 1200 说明书用户手册.PDF

10 x more sensitivity with the Agilent 1290/1260 Infinity Diode Array Detector compared to Agilent 1200 Series UV Detectors Technical Note Max-Light cartridge cell Introduction The sensitivity of ultraviolet (UV) detectors is the most important factor for many ana- lytical challenges. With the Agilent 1290/1260 Infinity Diode Array Detector (DAD), which incorporates the new Agilent Max-Light cartridge cell, it is now possible to increase sensitivity to more than ten times that of the Agilent 1200 Series UV detec- tors. This significant sensitivity increase simplifies sample preparation methods because small samples are needed for extraction and cleanup. In addition, lower lev- els of impurities such as genotoxins can be detected, in preparation for the possibility of more stringent regulatory requirements in the future. New optical design The Agilent 1290/1260 Infinity Diode Array Detector features a completely new optical design based on the Agilent Max-Light cartridge cell with optofluidic waveguides. This new cell technology uses the principle of total internal reflection along a noncoated fused silica capillary to increase the light transmission. Use of this technolo- gy achieves a new level of sensitivity without the decreased resolution that can be caused by cell dispersion effects. Furthermore, this flow cell Figure 1 design minimizes baseline perturba- Design of the Agilent 1290/1260 Infinity DAD. tions caused by the refractive index variations within the cell that are gen- Red: 1290 DAD, Blue: DAD SL erated by gradient analysis, tempera- mAU ture variations or solvent composition 30 Anthracene inhomogeneities. The stable base