Agilent The Agilent RNA Isolation Kit in Combination with New Agilent DNase Accessory kit Dramatically Reduces Genomic DNA Contamination of RNA Preparation说明书用户手册.PDF

The Agilent RNA Isolation Kit in Combina- tion with New Agilent DNase Accessory Kit Dramatically Reduces Genomic DNA Contamination of RNA Preparations Application Gene Expression Author accurate data if RNA samples are contaminated with significant amounts of genomic DNA (gDNA). Ilgar Abbaszade, PhD For example, due to intrinsic DNA polymerase Agilent Technologies, Inc. activity reverse transcriptase could label gDNA 2850 Centerville Road during the cDNA labeling reaction used for Wilmington, DE 19808-1610 microarray hybridization. In this case, the label in USA the form of a nucleotide triphosphate is incorpo- rated into partially degraded gDNA. As a result, false positive signals will be produced due to Abstract hybridization of the labeled gDNA to the array. In this application, as well as in many other applica- Real time PCR was used to quantify genomic DNA conta- tions such as real time PCR analysis, the gDNA mination in total RNA samples isolated with the Agilent could contribute to the positive expression signal Total RNA Isolation Kit, both alone and in combination and lead to data that does not reflect actual with the new Agil