Agilent Detection, Monitoring, and Quantitation of Trace Sulfonamides in Pork Muscle Using the Agilent 6410A LC MS MS 说明书用户手册.PDF

Detection, Monitoring, and Quantitation of Trace Sulfonamides in Pork Muscle Using the Agilent 6410A LC/MS/MS Application Brief Jian-qiu Mi Sulfonamides are the one of the oldest groups of veterinary medicines in use today. Highlights All sulfonamide drugs are currently included in Annex 1 of the Council Regulation 2377/90. The existing EU maximum residue level (MRL) for all drugs of the sulfon- • The pH of the mobile phase played amide group is 100 µg/kg in all food-producing species. an important role in the LC separa- tion because the retention behaviors A variety of methods have been used to measure sulfonamide residue in biological of the drugs were dependent on the materials, including thin-layer chromatography (TLC), high-performance liquid chro- ionization of the sulfonamides. matography (HPLC), high-performance capillary electrophoresis (HPCE), gas chro- matography (GC), and enzyme-linked immunosorbent assay (ELISA). Now, the • Different kinds of sulfonamide drugs LC/MS/MS method is used more widely. can be analyzed within one run. • Using the RRLC can get all 14 com- In this study, a multiresidue analysis was performed to simultaneously determine pounds to elute within 10 minutes. sulfonamides in pork by the Agilent 6410A LC/MS/MS. This multiresidue analysis for sulfonamides can detect different kind