Agilent Technologies Using DMSO as an Injection Solvent to Increase Sample Load in Preparative LC 应用笔记说明书用户手册.PDF

Using DMSO as an Injection Solvent to Increase Sample Load in Preparative LC Application Pharmaceuticals Authors uncover unexpected solubility problems, for exam- ple, peak broadening or peak splitting. This prob- Angelika Kaufmann and Ulrike Jegle lem may be resolved by adding the organic solvent Agilent Technologies DMSO (dimethylsulfoxide) to the injection solvent Waldbronn, Germany thereby improving sample solubility and Alexander Dreyer, Boehringer Ingelheim re-establishing good chromatography, that is, symmetrical peaks. Pharma GmbH Biberach, Germany Results Ronald E. Majors* Agilent Technologies, Inc. Some basic compounds, such as tertiary amines 2850 Centerville Road (see Figure 1), are difficult to separate and isolate Wilmington, DE 19808-1610 by HPLC because they tail badly on many silica- USA based HPLC columns. However, the ZORBAX Bonus-RP HPLC column incorporates an amide *Agilent contact linkage into the hydrocarbon bonded phase itself, shielding the secondary amines from unreacted Abstract