Agilent Mobile phase compensation method for the Agilent 1200 Series Evaporative Light Scattering Detector to significantly improve quantification of unknowns 说明书用户手册.PDF

Mobile phase compensation method for the Agilent 1200 Series Evaporative Light Scattering Detector to significantly improve quantification of unknowns Technical Note Introduction The Agilent 1200 Series Evaporative Light Scattering Detector (ELSD) detects and quantifies any compound that is less volatile than the mobile phase with and with- out available standards. It provides an essentially universal measurement under isocratic and gradient conditions and is independent of a compound´s absorbance, fluorescence, or electroactivity. Here, we show how mobile phase compensation using Agilent 1200 Series binary pumps significantly improves ELSD quantifications of unknowns by reducing the area relative standard deviation (RSD) for both signals, for example, UV from 11 to 0.83 (factor of 13.2) and ELSD from 34 to 1.7 (factor of 20). Mobile phase compensation method In gradient runs, quantifying unknowns using available standards can lead to errors due to differences in elution time between standard and unknown compounds. They can lead to different ELSD responses as the response depends on analytes organic content. The mobile phase compensation method is a method that compensates for changing organic content in gradient elution. In this method, a separate pump is attached post column that runs an opposite gradient to neutralize the effect of changing organic content. In this way, an isocratic flow reaches the detector. Factors influencing the ELSD response In the first stage of Agilent 1200 Series ELSD detection, the eluent is nebulized and small droplets are selected to minimize background noise. This is followed by an evaporation stage where solvents in the aerosol evaporate at low temperatures to avoid loss of compounds and form particles. Finally, in the detection stage, solute particles are focused, using gas-supported focusing (GSF) for enhanced signal-to- noise ratios and the intensity of scattered light from the particles is detect