DB43_T 2332-2022茶白星病原菌检测技术规程.docxVIP

ICS 67.140.10 CCS B 35 43 湖 南 省 地 方 标 准 DB43/T 2332—2022 茶白星病原菌检测技术规程 Technical regulation for detection of Elsinoe leucospila 2022 2022- 05 - 06 发布 2022- 08 - 06 实施 湖南省市场监督管理局 发 布 目 次 前言································ ································ ································ ························ Ⅲ 1 范围 ································ ································ ································ ····················· 1 2 规范性引用文件································ ································ ································ ······ 1 3 术语和定义 ································ ································ ································ ············ 1 4 样品采集 ································ ································ ································ ··············· 1 5 形态检测 ································ ································ ································ ··············· 2 6 分子检测 ································ ································ ································ ··············· 2 附录 A（资料性） 茶白星病的田间症状 ································ ································ ·········· 4 附录 B（资料性） 茶白星病菌的菌落、菌丝与孢子形态································ ····················· 5 附录 C（资料性） 茶白星病菌基因序列片段 ································ ································ ···· 6 参考文献································ ································ ································ ···················· 7 前 言 本文件按照 GB/T 1.1—2020《标准化工作导则 第 1 部分：标准化文件的结构和起草规则》的规定起草。 请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。本文件由湖南省农业农村厅提出。 本文件由湖南省农业农村标准化技术委员会归口。本文件起草单位：湖南省茶叶研究所。 本文件主要起草人：周凌云、杨辉、杨文波、向芬、刘红艳、李维、银霞、曾泽萱。 茶白星病原菌检测技术规程 范围 本文件规定了茶白星病原菌的术语和定义、样品采集、形态检测和分子检测。本文件适用于茶白星病菌-茶痂囊腔菌（Elsinoe leucospila）的检测。 规范性引用文件 下列文件中的内容通过文中的规范性引用而构成本文件必不可少的条款。其中，注日期的引用文件， 仅该日期对应的版本适用于本文件；不注日期的引用文件，其最新版本（包括所有的修改单）适用于本文件。 GB/T 6682 分析实验室用水规格和试验方法GB/T 8302 茶 取 样 SB/T 10314—1999 采样方法及检验规则 NY/T 3862 茶云纹叶枯病监测技术规程 3 术语和定义 3 术语和定义 本文件没有需要界定的术语和定义。 4 样品采集 采样特征 发生部位 茶白星病症状可见于 0～200