杏鲍菇多糖提取条件优化及其抗氧化、降血脂功能研究的开题报告.docxVIP

杏鲍菇多糖提取条件优化及其抗氧化、降血脂功能研究的开题报告 Title：Optimization of polysaccharides extraction condition from Pleurotus eryngii and its antioxidant and hypolipidemic activities research Introduction： Pleurotus eryngii, also known as king oyster mushroom or abalone mushroom, is a type of edible mushroom with high nutritional and medicinal values. Previous studies have reported that polysaccharides extracted from P. eryngii exhibit various biological activities, such as antioxidant, hypoglycemic, and hypolipidemic effects. Objective： The aim of this study is to optimize the polysaccharides extraction conditions from P. eryngii and investigate its antioxidant and hypolipidemic activities. Methodology： 1. Extraction optimization: Response surface methodology (RSM) will be employed to optimize the extraction conditions such as extraction temperature, extraction time, and water to P. eryngii ratio. The yield of polysaccharides will be used as the response variable. 2. Moreover, the properties of the obtained polysaccharides will be analyzed using high-performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FTIR). 3. Antioxidant activity evaluation: The antioxidant activity of the polysaccharides will be assessed using in vitro assays such as the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, the ferric reducing antioxidant power (FRAP) assay, and the total antioxidant capacity (TAC) assay. 4. Hypolipidemic activity evaluation: High-fat diet-induced hyperlipidemia rats will be used to evaluate the hypolipidemic effect of the polysaccharides. Blood samples will analyze biochemical parameters such as total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and malondialdehyde (MDA) levels. Significance： This study will provide new information about the optimal extraction conditions of polysaccharides from P. eryngii and its antioxidant and hypolipidemic activities. Th