厄多司坦预处理对大鼠心肌缺血—再灌注损伤的保护作用的中期报告.docx

厄多司坦预处理对大鼠心肌缺血—再灌注损伤的保护作用的中期报告 中文摘要： 本研究的目的是探究厄多司坦预处理对大鼠心肌缺血—再灌注(I/R)损伤的保护作用以及与过氧化氢+蒸馏水预处理的比较。 方法：40只雄性SD大鼠随机分为4组：对照组、I/R组、厄多司坦预处理组和过氧化氢+蒸馏水预处理组。大鼠在末端麻醉下通过左锁骨下动脉插管，制备LAD心肌缺血模型。术后24h，取心脏组织切片分析各组心肌梗死面积、心肌细胞凋亡、肿瘤坏死因子-α( TNF-α) 及超氧化物歧化酶(SOD)、丙二醛(MDA)的表达情况。 结果：与I/R组相比，厄多司坦预处理组心肌梗死面积明显减少，心肌细胞凋亡显著减少，TNF-α表达下降，SOD活性增加，MDA含量降低(P0.05)。与I/R组相比，过氧化氢+蒸馏水预处理组的心肌梗死面积、心肌细胞凋亡、TNF-α表达和SOD活性无显著差异(P0.05)。 结论：厄多司坦预处理可减轻大鼠心肌I/R损伤的程度，改善心肌细胞凋亡及抗氧化水平，其效果与过氧化氢+蒸馏水预处理相比更明显。 英文摘要： Objective: To investigate the protective effects of edaravone pretreatment on myocardial ischemia-reperfusion (I/R) injury in rats and compare them with hydrogen peroxide (H2O2) + distilled water pretreatment. Methods: 40 male SD rats were randomly divided into four groups: control group, I/R group, edaravone pretreatment group and H2O2 + distilled water pretreatment group. The LAD myocardial ischemia model was prepared by inserting a catheter through the left subclavian artery under terminal anesthesia. After 24 hours, heart tissues were collected for analysis of myocardial infarct size, myocardial cell apoptosis, tumor necrosis factor-α (TNF-α), superoxide dismutase (SOD) and malondialdehyde (MDA) expression. Results: Compared with the I/R group, the edaravone pretreatment group showed a significant reduction in myocardial infarct size, a significant decrease in myocardial cell apoptosis, a decrease in TNF-α expression, an increase in SOD activity and a decrease in MDA content (P0.05). There was no significant difference in myocardial infarct size, myocardial cell apoptosis, TNF-α expression or SOD activity between the H2O2 + distilled water pretreatment group and the I/R group (P0.05). Conclusion: Edaravone pretreatment can reduce the degree of myocardial I/R injury in rats, improve myocardial cell apoptosis and antioxidant levels, and its effect is more significant compared to H2O2 + distilled water pretreatment.