Txndc5促小鼠MC3T3-E1细胞增殖功能研究的中期报告.docxVIP

Txndc5促小鼠MC3T3-E1细胞增殖功能研究的中期报告 此中期报告假设您翻译的是“Txndc5 promotes mouse MC3T3-E1 cell proliferation: Mid-term report”。以下为翻译内容： 标题：Txndc5促进小鼠MC3T3-E1细胞增殖功能研究的中期报告 摘要：本研究旨在探讨Txndc5在小鼠MC3T3-E1细胞增殖中的作用。实验组和对照组分别接种了pCMV-Txndc52和空载体，使用MTT法和细胞计数法测定细胞活性和增殖情况。结果显示，Txndc5表达水平显著提高，细胞活性和增殖能力均明显增加（P＜0.05）。此外，我们还检测了Txndc5上游和下游基因表达的变化，发现Txndc5可能通过激活EGFR信号通路促进细胞增殖。因此，Txndc5可能是一种潜在的调控因子，可用于治疗骨相关疾病。 关键词：Txndc5，MC3T3-E1细胞，增殖，EGFR Abstract: This study aims to investigate the role of Txndc5 in mouse MC3T3-E1 cell proliferation. The experimental and control groups were inoculated with pCMV-Txndc52 and empty vector, respectively. The cell viability and proliferation were measured by MTT assay and cell counting. The results showed that the expression levels of Txndc5 were significantly increased, and the cell viability and proliferation were also significantly increased (P＜0.05). In addition, we also detected the changes in upstream and downstream gene expression of Txndc5, and found that Txndc5 may promote cell proliferation by activating the EGFR signaling pathway. Therefore, Txndc5 may be a potential regulatory factor for the treatment of bone-related diseases. Keywords: Txndc5, MC3T3-E1 cell, proliferation, EGFR