文案人类线粒体直接转移基于内共生理论新概念.pdfVIP

DirectHumanMitochondrialTransfer:ANovelConceptBasedonthe

EndosymbioticTheory

abbaab,

T.Kitani,D.Kami,T.Kawasaki,M.Nakata,S.Matoba,andS.Gojo*

aDepartmentofCardiovascularMedicine,GraduateSchoolofMedicalScience,KyotoPrefecturalUniversityofMedicine,Kyoto,Japan;

bDepartmentofRegenerativeMedicine,GraduateSchoolofMedicalScience,KyotoPrefecturalUniversityofMedicine,Kyoto,Japan

Mitochondriayanessentialroleineukaryotes,andmitochondrialdysfunctionis

implicatedinseveraldiseases.Therefore,intercellularmitochondrialtransferhasbeen

proposedasamechanismll-basedtherapy.Inaddition,internalizationofisolated

mitochondriacellsbysimplecoubationwasreportedtoimprovemitochondrialfunctionin

therecipientcells.However,substantialevidenceforinternalizationofisolatedmito-

chondriaisstilllacking,anditsprecisemechanismremainselusive.Wetestedwhether

enrichedmitochondriacanbeinternalizedintoculturedhumancellsbysimplecouba-tion

usingfluorescencemicroscopyandflowcytometry.Mitochondriawereisolatedfrom

endometrialglandderivedmesenchymalcells(EMCs)orEMCsstablyexpressing

mitochondrial-targetedredfluorescentprotein(EMCs-DsRed-mito),andenrichedbyanti-

mitochondrialantibody-conjugatedmicrobeads.Theywerecoubatedwithisogeneic

EMCsstablyexpressinggreenfluorescentprotein(GFP).Livefluorescenceimagingclearly

showedthatDsRed-labeledmitochondriaaccumulatedinthecytosmofEMCsstably

expressingGFParo