研究重组人内皮抑素恩度抑制小鼠结肠癌异种移植模型中肿瘤生长转介绍.pdfVIP

Pathol.Oncol.Res.(2012)18:315–323

10.1007/s12253-011-9447-y

RESEARCH

RecombinantHumanEndostatinEndostarInhibitsTumor

GrowthandMetastasisinaMouenograftModel

ofColonCancer

YitaoJiaMinLiuWangangHuangZhenbaoWangYutongHeJianhuaWu

ShuguangRenYingchaoJuRuichaoGengZhongxinLi

Received:7December2010/Accepted:1August2011/Publishedonline:22September2011

#TheAuthor(s)2011.Thisarticleispublishedwithopenaccessat

Toinvestigatetheeffectsofrecombinanthumanaroundthetumorareawas12.27±1.21and22.25±2.69per

endostatinEndostaronmetastasisandangiogenesisandfieldinEndostar-treatedmiceandcontrols(P0.05),

lymphangiogenesisofcolorectalcancercellsinamouserespectively.Endostaralsodecreasedthedensityoflym-

xenograftmodel.ColoncancercellsSW620wereinjectedphaticvesselsintumortissues(7.84±0.81vs.13.83±1.08,

subcutaneouslyintothelefthindflankofnudemicetoP0.05).Endostarsuppressesangiogenesisandlymphangio-

establishmouenograftmodels.Themiceweretreatedgenesisinthelymphnodeswithmetastases,simultaneously.

withnormalsalineorEndostarsubcutaneouslyeveryotherTheexpressionofVEGF-A,-Cand-DinSW620cellstreated

day.Thegrowthandlymphnodemetastasisoftumorcells,withEndostarwassubstantiallylowerthanthatofcontrols.

angiogenesisandlymphangiogenesisintumortissuewereEndostarinhibitedgrowthandlymphnodemetastasisof

detected.Apoptosisandcellcycledistributionwerestudiedbycoloncancercellsbyinhibitingangiogenesisandlymphan-

flowcytometry.Theex