Bis-Tris-gel配方近年原文.docVIP

TomakeBis-Trisgelwith1.5mmcombs

8%

12%

Separatinggel

1gel

2gels

4gels

6gels

8gels

1gels

2gels

4gels

Milli-Qwater

3.7ml

7.4ml

14.8ml

22.2ml

29.6ml

2.7ml

5.4ml

10.8ml

3.5×Bis-Trisgelbuffer

2.3ml

4.6ml

9.2ml

13.8ml

18.4ml

2.3ml

4.6ml

9.2ml

32%Acrylamide-Bisgel

2.0ml

4.0ml

8.0ml

12ml

16ml

3.0ml

6.0ml

12ml

10%APS

75ul

150ul

300ul

450ul

600ul

50ul

100ul

200ul

TEMED

5ul

10ul

20ul

30ul

40ul

3ul

6ul

12ul

Total

8ml

16ml

32ml

48ml

64ml

8ml

16ml

32ml

Stackinggel

1gel

2gels

4gels

6gels

8gels

Milli-Qwater

1.76ml

3.52ml

7.04ml

10.56ml

14.08ml

3.5×Bis-Trisgelbuffer

0.86ml

1.72ml

3