NAT10作为肝癌潜在生物学标志物的生物信息学分析.pdfVIP

医学研究杂志2025年3月第54卷第3期·论著·

NAT10作为肝癌潜在生物学标志物的生物信息学分析

王丹姜可伊付松波孙悦

摘要目的探讨NAT10在肝癌中的临床意义与潜在作用机制。方法基于癌症基因组图谱(TheCancerGenomeAtlas,

TCGA)获得的50例正常和374例肿瘤样本,采用R4.2.1处理所获数据。结果NAT10在肿瘤样本中的表达显著高于正常样

本(P0.001)。与NAT10低表达患者比较,NAT10高表达患者预后较差(P0.001)。Logistic回归分析结果表明,NAT10高表

达与肝癌患者临床预后不良因素相关,NAT10高表达患者更易于进展到晚期。基因集富集分析(genesetenrichmentanalysis,

GSEA)结果显示,NAT10高表达样本中,异生物质代谢、凝血、脂肪酸代谢等相关基因特征均有差异富集。蛋白互作分析结果显

示,NAT10可能与IGF2、SST、MUC2、CHGA、AGR2等基因存在相互作用。结论NAT10在肝癌中高表达,且表达水平与肝癌的临

床特征及患者生存率存在相关性。NAT10可能是一种潜在的肝癌预后生物学标志物。

关键词NAT10肝癌预后不良生物学标志物

中图分类号R735文献标识码ADOI10.11969/j.issn.1673-548X.2025.03.010

BioinformaticsAnalysisofNAT10AsAPotentialBiomarkerforHepatocellularCarcinoma.WANGDan,JIANGKeyi,FUSongbo,et

al.InstituteofKeshanDisease,CenterforEndemicDiseaseControl,ChineseCenterforDiseaseControlandPrevention,HarbinMedicalU-

niversity,Heilongjiang150081,China

AbstractObjectiveToinvestigatetheclinicalsignificanceandpotentialmechanismofNAT10inhepatocellularcarcinoma.Meth-

odsAtotalof50normaland374tumorsampleswereobtainedbasedonTheCancerGenomeAtlas(TCGA),andthedatawerepro-

cessedbyR4.2.1.ResultsTheexpressionofNAT10intumorsampleswassignificantlyhigherthanthatinnormalsamples(P

0.001).ComparedwithpatientswithlowexpressionofNAT10,patientswithhighexpressionofNAT10hadworseprognosis(P

0.001).LogisticregressionanalysisshowedthathighNAT10expressionwasassociatedwithadverseclinicalprognosticfactors,andpa-

tientswithhighNAT10expressionweremorelikelytoprogresstotheadvancedstage.Genesetenrichmentanalysis(GSEA)results

showedthatthegenesrelatedtoheterobioticmetabolism,bloodcoagulationandfattyacidmetabolismweredifferentiall