气相层析质谱仪-瓦里安科技.pptxVIP

氣相層析質譜儀

GC/MS美商亞洲瓦里安科技(股)公司

GC基本原理MS基本原理數據處理定性定量MSTuning與日常保養應用

第一部分GC基本原理

GC基本組件Injector------CapillaryInjectorColumn------CapillaryDetector------MassRecorder------Computer

GC簡圖

CapillaryGCReview

UniversalorSelective

GCDetectorsFlameIonizationDetector(FID)forHydrocarbonCompoundsThermalConductivityDetector(TCD)UniversalDetectors01ElectronCaptureDetector(ECD)PulsedFlamePhotometricDetector(PFPD)ThermionicSpecificDetector(TSD)SelectiveDetectors02

InformationFromGCRetentionTimeSamecompoundhasthesameR.T.underthesameGCconditions.Qualitation01PeakAreaPeakHeightQuantitation02

第二部分MS基本原理

TheBasicPrincipleof

MassSpectrometryMM+.M+.m/z15m/z57m/z27m/z43m/z29MassAnalysisDataRecordingPlotofBarGraph2943IonizationMassAnalyzerIonDetectionCH3-CH2-CH2-CH3

TheBasicPrincipleof

MassSpectrometryCH3-CH2-CH2-CH3CH3-CH2-CH2-CH3CH3-CH2-CH2-CH3em/z58m/z43m/z2912345

InletSystems01GC,LC,DirectProbeInlet…..IonSource02EI,CI……MassAnalyzer03MagneticSector,TheQuadrupoleIonTrap…..Detector04ElectronMultiplier…..MassSpectrometry

Ionization:

ElectronImpactM+.m/z15m/z57m/z27m/z43m/z294358

Ionization:

ChemicalIonizationCH4+e-→CH4+?+2e-CH4+?+CH4→CH3?+CH5+CH5++M→CH4+(MH)+CH4:CIgasM:Analyte

ElectronImpactChemicalIonization

MassAnalyzer:

MagneticSector

MassAnalyzer:

ExternalSource/Quadrupole

MassAnalyzer:

TheQuadrupoleIonTrapTopendcapBottomEndcapRingElectrodeGCcolumninletFilamentassemblyIonMultiplier

DetectorFo

IonDetection:

ElectronMultiplier1Sn/SnOorPb/PbOdopedglassfunnel2Showerofelectronsisreleasedwhenanionstrikesthewall3Theamplifiedioncurrentissenttotheelectroniccircuitandconvertedtoavoltagesignal4105~107gain

DiscreteDynodeElectronMultiplierIonbeamslitelectronsToamplifier

ContinuousDynodeElectronMultiplierCascadeofelectronsResistiveconductivesurfaceToamplifier2kV

GCMass

WhenMS

BecomesaGCDetector

GC/MS