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AHRagonist10

Cat.No.:HY-178053

CASNo.:1191937-43-5

分⼦式:C₁₈H₂₀O₃

分⼦量:284.35

作⽤靶点:ArylHydrocarbonReceptor;CytochromeP450

作⽤通路:Immunology/Inflammation;MetabolicEnzyme/Protease

储存⽅式:Pleasestoretheproductundertherecommendedconditionsin

theCertificateofAnalysis.

BIOLOGICALACTIVITY

⽣物活性AHRagonist10是⼀种强效的AHR激动剂(EC50=2.01nM)。AHRagonist10能够提⾼关键AHR下游通路靶

基因的转录⽔平，包括CYP1A1和CYP1B1。AHRagonist10能够下调CD36和IL-18的表达⽔平，并且对正

常细胞显⽰出较低的细胞毒性(40μM)。AHRagonist10可以抑制CCL5、CCL20、IL-6、IL-8、S100A9、

TLR4、TNF-α和TNFR1的表达，表明AHRagonist10通过AHR依赖性信号通路有效调节炎症反应。AHR

agonist10可⽤于银屑病研究[1]。

体外研究AHRagonist10(10μM,20μM,24h)caneffectivelyenhancetheexpressionlevelsofCYP1A1andCYP1B1

intheAHRknockdownHaCaTcells[1].

AHRagonist10(5μM)cansuppresstheexpressiononthemRNAlevelofCYP1A1,CYP1B1,CCL5,

CCL20,IL-6,IL-8,S100A9,TLR4,TNF-α,andTNFR1ontheLipopolysaccharides(HY-D1056)(LPS)-

inducedHaCaTcell[1].

RT-PCR[1]

CellLine:AHRknockdownHaCaTcell

Concentration:10μM,20μM

IncubationTime:2