1株asdA缺陷型减毒鼠伤寒沙门氏菌作为核酸疫苗载体的构建与鉴定(英文).docxVIP

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1株asdA缺陷型减毒鼠伤寒沙门氏菌作为核酸疫苗载体的构建与鉴定(英文)

一、Introduction

1.Backgroundofthestudy

(1)Thedevelopmentofeffectivevaccinesiscrucialforthepreventionandcontrolofinfectiousdiseases.Overthepastfewdecades,traditionalvaccineshaveplayedapivotalroleinreducingtheincidenceandseverityofmanyinfectiousdiseases.However,theemergenceofnewandemergingpathogens,alongwiththechallengesposedbyantibioticresistance,necessitatestheexplorationofnovelvaccinetechnologies.Amongthese,DNAvaccineshaveemergedasapromisingapproachduetotheirabilitytoelicitrobustimmuneresponsesandtheirpotentialforrapiddevelopmentandcustomization.

(2)Salmonellatyphimurium,anon-pathogenicstrainofSalmonella,hasbeenextensivelystudiedasavaccinevectorduetoitsuniqueproperties.Itishighlyimmunogenic,capableofreplicatingwithinhostcells,andcanbeeasilygeneticallymanipulated.OneofthemostattractivefeaturesofSalmonellaasavaccinevectorisitsabilitytoinduceastrongimmuneresponsewithoutcausingdisease.Thisisparticularlysignificantforthedevelopmentofliveattenuatedvaccines,wherethesafetyandefficacyofthevaccineareofutmostimportance.

(3)TheASDA(adenosinedeaminase)geneisacriticalvirulencefactorinSalmonella.MutationsintheASDAgenehavebeenshowntoreducethepathogenicityofSalmonella,makingitanattractivecandidateforthedevelopmentofsafeandeffectiveliveattenuatedvaccines.TheconstructionofanASDA-deficientSalmonellastrainasavaccinevectorhasseveraladvantages.Firstly,itcanenhancethesafetyofthevaccinebyreducingtheriskofdiseasecausedbythevectorstrain.Secondly,itcanimprovetheefficacyofthevaccinebyenhancingtheimmuneresponsetotheantigenofinterest.Finally,itcansimplifytheproductionprocessbyeliminatingtheneedforcomplexpurificationsteps.

2.Significanceoftheresearch

(1)Thesigni