基于菌属特异性抗体的金标试纸传感器同时检测12种沙门氏菌（英文）.docxVIP

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基于菌属特异性抗体的金标试纸传感器同时检测12种沙门氏菌（英文）

一、Introduction

1.Backgroundof沙门氏菌检测

Salmonellainfections,causedbythegenusSalmonella,areasignificantpublichealthconcernworldwide.Thesebacteriaareknowntocausegastrointestinalillnessinhumans,rangingfrommildsymptomssuchasdiarrhea,fever,andabdominalcrampstoseverecasesthatcanleadtohospitalizationandevendeath,particularlyinvulnerablepopulationsliketheelderly,youngchildren,andindividualswithweakenedimmunesystems.ThedetectionofSalmonellainfoodandwateriscrucialforpreventingoutbreaksandcontrollingthespreadofthedisease.

ThedetectionofSalmonellahastraditionallyreliedonlaboratory-basedmethodsthatinvolveculturingthebacteriainacontrolledenvironment.Thesemethods,whilereliable,aretime-consuming,requiringseveraldaystoobtainresults.Moreover,theyrequirespecializedequipmentandtrainedpersonnel,whichcanbelimitinginresource-poorsettings.Overtheyears,therehasbeenagrowinginterestindevelopingrapid,sensitive,andcost-effectivediagnostictoolsthatcanbeusedinpoint-of-caresettings.Thesetoolsareessentialforearlydetectionandtimelytreatment,whichcansignificantlyreducetheseverityanddurationofinfections.

Advancementsinmolecularbiologyhaveledtothedevelopmentofnucleicacid-baseddetectionmethods,suchaspolymerasechainreaction(PCR),whichofferhighsensitivityandspecificity.However,thesemethodsoftenrequireexpensiveequipmentandskilledoperators,whichcanlimittheirwidespreaduse.Furthermore,thereisaneedformultiplexingcapabilitiestodetectmultipleSalmonellaserotypessimultaneously,asthegenusincludesover2,500differentserotypes,eachwithvaryingvirulenceanddiseaseoutcomes.ThismultiplexingchallengenecessitatesthedevelopmentofinnovativedetectionplatformsthatcanefficientlydistinguishbetweendifferentSalmonellaspeciesand