《单分子的光学探测与应用》教学课件PPT教案-2物理原理与测量.pptVIP

**三重态tripletstateT1isa“dark”metastablestate（亚稳态）Whentheemissionstops,detectedphotonsoriginatefromthebackgroundS0S1T1PISCtTWindowsizeW=100binperiod=50msFluorescencemonitoring**Fig.2.40mssectionsoftotalemissiontimetraceforthreedifferentmoleculesonthesamesample.On-stateaveragecountsNonandoff-statelifetimeτoffareshownfortwomoleculesinbandc.**DataanalyzedThedatawasanalyzedinthefollowingway:Foreachevent(transitionfromonstatetooffstate),wemeasuredthenumberofcountsintheonsta