留兰香提取物体外抗菌作用初步研究.pdfVIP

中国农学通报 2010,26(01): 1-4 Chinese Agricultural Science Bulletin 留兰香提取物体外抗菌作用初步研究 韩卫丽，崔保安，张红英 （河南省动物性食品安全重点实验室，河南农业大学牧医工程学院，郑州450002 ） 摘 要：【目的】研究留兰香提取物的体外抗菌作用。【方法】采用管碟法测定留兰香提取物抑菌圈大 小，采用试管2 倍稀释法测定留兰香提取物对4 种动物病原菌的最低抑菌浓度(MIC) ，判定抗菌效 果。【结果】留兰香水煎剂、醇提物、挥发油对4 种动物病原菌有不同程度的抗菌作用，尤其是对猪葡萄 球菌抑制效果比较明显，留兰香水煎剂Ⅰ的MIC 为31.25 mg/ml ，醇提物Ⅲ的MIC 为62.5 mg/ml ，挥 发油Ⅴ的MIC 为2.32 mg/ml ，挥发油Ⅵ的MIC 为2.31 mg/ml 。【结论】留兰香水煎剂、醇提物及挥发油 体外有一定抑菌作用，不同提取方法所得提取物对试验菌抑制效果不同。 关键词：留兰香；提取物；最低抑菌浓度 中图分类号：S853.7 文献标识码：A 论文编号：2009- 1590 Initial Study on Anti-bacterial Activity of Mentha spicata Linn Extraction in vitro Han Weili, Cui Baoan, Zhang Hongying (College of Animal Husbandry and Veterinary, Henan Agricultural University, Zhengzhou 450002) Abstract: 【OBJECTIVE 】To investigate the antibacterial function of Mentha spicata Linn extracts on Escherichia coli, Salmonella, Enterococcus, Staphylococci in vitro. 【METHOD 】In the study, the agar Cup-plate method was used to mensurate the size of antibacterial zone of Mentha spicata L. Extractions and the tube doubleness dilution method were used to determine their Minimum inhibitory concentra⁃ tions (MIC) for 4 animal bacteria strains. 【RESULTS 】The aqueous extract, the alcohol extract and the volatile oil of Mentha spicata L. Has inhibitory effect to the selected strains. All of them have the best inhibitory effect on Staphylococci. The MIC of aqueous extract Ⅰ(sinking with alcohol) is 31.25mg/ml. The MIC of alcohol extraction Ⅲis 62.5mg/ml. The MIC of volatile oil Ⅴis 2.32mg/ml. The MIC of vol⁃ atile oil Ⅵis 2.31mg/ml. 【CONCLUSION 】Mentha spicata L. shows anti-bacterial effects on the experi⁃ mental str