柱层析法分离纯化菊粉酶的研究.pdfVIP

沈阳农业大学学报袁圆园10原08 袁41穴4雪院484-487 允燥怎则灶葬造 燥枣 杂澡藻灶赠葬灶早 粤早则蚤糟怎造贼怎则葬造 哉灶蚤增藻则泽蚤贼赠袁圆园10原08 袁41穴4雪院484-487 柱层析法分离纯化菊粉酶的研究 a b c b 晓颖 李秉超 *,林荣峰 李丽梅 沈阳农业大学a.食品学院,b.理学院袁c.畜牧兽医学院沈阳 110866冤 摘要 黑曲霉固体发酵法产生的粗菊粉酶为试材袁采用葡聚糖凝胶层析法和DEAE 纤维素52 柱层析法对经过硫酸铵沉淀后的 粗酶进行了分离纯化袁得到接近电泳纯的高纯度菊粉酶遥结果表明院 菊粉为底物研究酶活时袁DEAE-纤维素52 离子交换层析得 到较好的分离效果袁 主要活力峰比活力 12.64U 窑mg-1 提纯了6.37 倍曰 蔗糖为底物时袁SephadexG100 分离效果好袁 比活力 35.57U 窑mg-1 提纯了3.78 倍遥 关键词 菊粉酶曰Sephadex 凝胶层析曰DEAE-纤维素离子交换层析 中图分类号 Q533 文献标识码 A 文章编号 1000- 1700 渊20 10冤04-0484-04 Column Chromatography Purification of Inulinase a b c b NI Xiao-ying 袁 LI Bing-chao *袁 LIN Rong-feng , LI Li-mei (a. College of Food Science, b.College of Science, c.College of Animal Husbandry and Veterinary, Shenyang Agricultural University,Shenyang 110866, China) Abstract 院 With the inulinase produced by solid fermentation of A sp ergillus niger as test material, crude enzyme was purified after ammonium sulfate precipitated by Sephadex chromatography and DEAE-cellulose 52 column chromatography, and then high purity inulinase close to electrophoretic purity was abtained. The results showed that when inulin was as the substrate of activity, DEAE -cellulose 52 ion exchange chromatography achieved good separation, and the main activity peak specific activity was 12.64 U 窑mg -1 with 6.37 -fold purification; when sucrose was as the substrate, SephadexG100 achieved good separation,and