Chapter 22 肝炎病毒.pptVIP

Chapter 22   3.Immunity Ⅲ.Laboratory Diagnosis  4.Compositions of antigen Hepatitis B surface antigen (HBsAg) Hepatitis B core antigen(HBcAg) Hepatitis B e antigen(HBeAg) ? HBcAg ⑴Locate in surface of viral core ⑵Anti-HBc+---marker of viral infection and replication ? HBeAg ⑴Exist in HBsAg-positive serum ⑵Associated with presence of Dane particles and DNAP ⑶HBeAg+---marker of virus replication and high infectivity of serum ⑷Anti-HBe+ ----marker of lower infectivity 5.Animal model and cell culture ? Source of infection 2.Immunity Specific humoral immunity Specific cellular immunity * * pathogen of viral hepatitis Introduction 1. Human hepatitis viruses ? HAV(+ssRNA) , HBV(dsDNA), HCV(+ssRNA) , HDV(–ssRNA) and HEV(+ssRNA) ? HGV(+ssRNA) and TTV(–ssDNA) 2.Other viruses of causing viral hepatitis (CMV,EBV,Rubella virus,VZV,Yellow fever virus,etc.) *pathogen of hepatitis type A *classified as Hepatornaviridae (肝RNA病毒属) 1. Shape and structure ?spherical particle(d=27nm) ?icosahedral nucleocapsid ?ssRNA (positive stranded) ?can code for polyprotein,RNA polymerase and proteinase VP1-4 ? Genome ? Naked virus ↓ Cleave 4 polypeptide (VP1 to VP4) ↓ capsomere→capsid capsomere Ⅰ.Biological Properties ? infective model(黑猩猩、狨猴、红面猴) ? cell culture (原代肝细胞或恒河猴胚肾传代细胞) ? stable to treatment with ether(乙醚), acid (PH3) and heat (60℃ for 1 hour) ? tolerate boiling for 5 minutes 2.Resistance 3.Cultivation (multiply slowly, non-CPE) ?Antigenicity of capsid protein:HAV-Ag (only one serotype) 1.Pathogenicity ? Source of infection ? Route of transmission ?asymptomatic or only mildly ill ?acute hepatitis of short incubation ?fulminant hepatitis and favorable prognosis ? Feature of HAV-infection by fecal-oral route (通过粪-