阿魏菇论文：新疆阿魏菇多糖对小鼠宫颈癌U14荷瘤鼠免疫功能影响的研究.docVIP

阿魏菇论文：新疆阿魏菇多糖对小鼠宫颈癌U14荷瘤鼠免疫功能影响的研究 【中文摘要】从肿瘤免疫学角度探讨阿魏菇多糖抑制宫颈癌的作用机理。方法：1.建立昆明小鼠宫颈癌U14细胞移植瘤动物模型,随机分为模型对照组,顺铂为阳性对照组,阿魏菇多糖组,阿魏菇多糖+顺铂组4组,分别给予不同的药物干预10天：2.检测抑瘤率,同时观察阿魏菇多糖对小鼠免疫器官脾脏的影响；3.鸡红细胞半体内法观察阿魏菇多糖对小鼠腹腔巨噬细胞吞噬功能的影响；4.MTT法测定阿魏菇多糖对小鼠脾脏中NK细胞影响；5.淋巴细胞转化实验检测阿魏菇多糖对小鼠脾脏T、B淋巴细胞的影响；6.采用ELISA法检测阿魏菇多糖对荷瘤小鼠血清中肿瘤坏死因子-α(TNF-α)与干扰素-γ(IFN-γ)水平的影响；7.流式细胞仪检测荷瘤鼠外周血T细胞亚型。8.采用SPSS13.0统计软件进行统计学处理,两组指标比较采用t检验。结果：阿魏菇多糖对荷瘤鼠细胞免疫的影响：1.阿魏菇多糖可提高荷瘤小鼠腹腔MΦ的吞噬功能,吞噬百分率和吞噬指数均高于模型对照组,差异有统计学意义(p0.05)。当两药合用时CD4+/CD8+比值较单用顺铂时降低,但无统计学意义。阿魏菇多糖对荷瘤鼠分子免疫的影响：分别与模型对照组及顺铂组相比,阿魏菇多糖组和两药合用组荷瘤鼠血清中细胞因子TNF-α和IFN-γ的水平均增高(p0.05),但模型对照组同顺铂组之间差别无统计学意义。当两药合用时,荷瘤小鼠血清中TNF-α水平高于单用顺铂组(p0.05),但IFN-γ水平提高不显著。抑瘤率：阿魏菇多糖组、顺铂组及两药联合组的瘤重与模型对照组瘤重相比均减轻,差异有统计学意义(P0.01),且两药联合组瘤重较顺铂组减轻,差异有统计学意义(P0.05)；各给药组抑瘤率分别为：39%、46.5%、65.1%。结论：1.阿魏菇多糖可通过增强巨噬细胞吞噬功能、NK细胞杀伤活性、T、B淋巴细胞的活性而提高宫颈癌移植瘤U14小鼠的细胞免疫功能。2.阿魏菇多糖可促进免疫活性细胞分泌TNF-α和IFN-γ水平,而发挥增强荷瘤鼠分子免疫的功能。3.阿魏菇多糖可使荷瘤鼠CD4+/CD8+比值恢复接近正常,说明其具有调节机体生理功能平衡的特点。.4.阿魏菇多糖抑瘤率达39%,当两药联用时,抑瘤率达65.1%,上述免疫指标较单用顺铂时均有不同程度增高,说明两药合用可改善顺铂对机体造成的免疫抑制,并且可以提高顺铂的抗肿瘤疗效。 【英文摘要】:To investigate polysaccharides from Pleurotus ferulae (PFP) for their regulative effects on Immune System and murine uterocervical carcinoma number14(U14).Methods:Animal tumor models have been established, then ramdomly divided them into 4 groups. Model group, DDP group, PFP group and PFP+ DDP group, given various treatment for 10 days; After treatment, the weight of the tumor was observed to evaluate the growth inhibitory effect of PFP. The effect of PFP on the proliferation and transformation of lymphocytes in spleen was determined by MTT assay, the effect of PFP on the killing activity of NK cell was determined by lactate dehydrogenase releasing assay. Mouse peritoneal macrophages phagocytosis of chicken red blood cell (semi-in vivo method) test. The tumor weight was measured after treatment. The expression of TNF-a and IFN-y protein in Peripheral blood were assayed by ELISA. The blood T lymphocyte subsets were assayed by using immunofluorescence staining and fl