罗布麻叶提取物对两肾一夹加高糖高脂饮食大鼠肾性和肥胖型高血压的保护作用01.doc

Prevention of renal and obesity-related hypertension in 2K1C rats on high-fat and refined-carbohydrate diet by Apocynum venetum L. leaf extract Xiang-qi Wu1 2, Xin-bo Yang*1, Jie-ren Yang2,Zheng-ming Huang3, Hong-yan Chen1, Miao Geng1,Jian-hua Wang1 (1Institute of Geriatrics, General Hospital of PLA, Beijing 100853 China) (2Dept of Pharmacology, Wannan Medical College,Wuhu Anhui 241001 China) (3Dept of Pharmacy, Hospital 302 of PLA, Beijing 100853 China) Abstract: AIM: To observe antihypertensive effects of Apocynum venetum L. leaf extract(ALE) and explore its part mechanism in 2-kidney, 1-clip (2K1C) rats on high-fat and refined-carbohydrate diet. METHOD: Rats with metobolic syndrome were copied by two-Kidney, one-Clip method and high-fat and refined-carbohydrate diet for twelve weeks. Systolic blood pressure(SBP) was determined at 5th week, forty-two rats with SBP exceeding 160mmHg were randomly assigned to five groups: ALE with high-, middle- and low-dose groups(200, 100, 50mg·kg-1·d-1, n=8), captopril group(30 mg·kg-1·d-1, n=8) and model group(0.5%carboxymethylcellulose 5 ml·kg-1·d-1, n=10), another laying sham-operated group(0.5%carboxymethylcellulose 5 ml·kg-1·d-1, n=10). Rats were intragastric administrated between 9:00-10:00 everyday for eight weeks according to above-mentioned setting dosage. Body weight was recorded weekly, and the dose was adjusted adcording to weight changes. At 5th, 9th and 13th week, SBP and urinary volume for 24 hours were determined. At 13th week, body weight, left clipped kidney weight and white adipose tissue humid weight(WHW) were weighed, oxidative and antioxidative indexes for clipped kidney tissue, levels of angiotensin Ⅱ and aldosterone for plasma, content of insulin, leptin, TNF-α, IL-6, TG, TC, Scr and BUN for serum were measured. Pathological changes of liver tissue was observed by hematoxylin-eosin staining method. RESULTS:ALE treatment significantly reduced established hypertension at 9th and 13th week. The level of oxid