医学微生物学英文版课件_26.pptVIP

Laboratory Diagnosis Category of Sample Blood, Urine, Stool, nasal washing, nasal swab , throat swab, saliva , sputum, rectal swab, vesicle fluid( scraping or swab), tissue ,brain biopsy, cerebrospinal fluid, et al. Laboratory Diagnosis Microscopy Identification Virus isolation and identification Detection of viral proteins( antigens and enzymes)] Detection of viral genetic material Serologic procedures Microscopy Identification Light microscopy Fluorescent microscopy Electron microscopy Light microscopy Characteristic CPE Inclusion Bodies Cell death Cell rounding Degeneration Aggregation Loss of attachments to substrate Characteristic histological changes:inclusion bodies in the nucleus or cytoplasm, margination of chromatin Syncytia: multinucleated giant cells caused by virus-induced cell-cell fusion Fluorescent microscopy Fluorescent-antibody staining Electron microscopy Direct detection : Human rotavirus; HAV; HBV; Smallpox virus; Herpes virus. Immune Electron microscopy: Human rotavirus; HAV; Laboratory Diagnosis Microscopy Identification Virus isolation and identification Detection of viral proteins( antigens and enzymes)] Detection of viral genetic material Serologic procedures Viral isolation and Identification Viral Growth and Cell culture Viral Detection Viral Identification Interpretation of culture results Systems for the Propagation of Viruses People Animals: cows, chickens, mice,rats, suckling mice Embryonated eggs Organ and tissue culture Organ culture Primary tissue culture Cell lines: diploid Tumor or （immortalized ）cell line Viral detection CPE Hemadsorption Interfere Metabolize of cell TCID50(Tissue culture infective dose) TCID50 is defined as that dilution of virus which will cause CPE in 50% of a given batch of cell culture TCID50= log10 of highest dilution giving 100%CPE +1/2 – (total number of test units showing CPE)/ (number of test units per dilution) Viral identification Complement fixation：