微生物浊度法测定硫酸庆大霉素颗粒的效价.pdfVIP

2009 S ep, 4( 3) Journ al of Ch engdu M edical Coll ege 205 doi: 101 3969/ j issn 1674- 22571 20091 031 0 13 # 论 著# 汪 洋, 闵 盛 ( , 6 10045) = , , , ; , , = ; ; ; = R9271 2 = A = 1674-2257( 2009) 0 3- 0205- 03 Determination of titer of gentamycin sulfate granules by turbidimetric method WAN G Yang ,MIN Sheng (Food and Drug Insp ection and Testing Center of Chengdu City , Chengd u 610045, China) = Abstract Objective T o establish a m ethod f or the det erm inatio n of the t iter of g entam ycin sulf ate g ranules Methods T urbidim etr ic m ethod w as u sed to determ ine the titer of g entamy cin sulf at e g ranules and the f inding s w ere comp ared w ith those detected by diffusion m etho d T urbidim etr ic m ethod w as subj ected to m etho do log y verif icatio n sub seqently Results L og arithm of co ncent ration w as linear w it h absor b abilit y and the averag e r eco very r ate w as 1001 3% by using turbidim etr ic m etho d T here w ere no signif icant diff erences in determ ination r esult s betw een tur bi- dim et ric m ethod and diffu sion m ethod M ethodolo gy v erif icat ion demon st rated th at tur bidim et ric metho d w as f easi- ble Conclusion T urbidim etric m ethod is pro ved to be co nv enient , accurat e and r apid f o r determ ination of the titer of g entamy cin sulf at e g ranules = Key words m icro biolog ical assay of antibiot ics; t ur bidim et ric m ethod ;