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Isolation, identification and characterization of human
intestinal bacteria with the ability to utilize chloramphenicol
5
10
15
20
25
30
35
40
45
as the sole source of carbon and energy#
Zhao Xin1, Tian Fengwei1, Wang gang1, Liu Xiaoming1, Zhang Qiuxiang1,
Zhang Hao1, Chen Wei2**
(1. School of Food Science and Technology, Jiangnan University, JiangSu WuXin 214122;
2. State Key Laboratory of Food Science and Technology, Jiangnan University,
JiangSu WuXin 214122)
Abstract: Five aerobic intestinal bacterial strains that utilized chloramphenicol (CAP) as sole carbon
and energy source were isolated from fecal samples collected from healthy volunteers. Based on their
morphology, physiological characters, and 16S rDNA sequence analysis, four of the five strains were
identified as Klebsiella pneumonia and one as Escherichia fergusonii. The degradation rate of strain
I-10-CHL (E. fergusonii) varied with the initial concentration of CAP and the maximum specific
substrate removal rate was observed at 25 μg CAP/ml. The pH value also had an effect on the
degradation rate of CAP and bacterial growth. A pH of 6.5 was optimal for CAP degradation and
growth of strain I-10-CHL (E. fergusonii). In mixed substrate batch cultivations, where CAP was one
of the components, glucose, acetamide and CAP were simultaneously utilized. The presence of glucose
and acetamide increased the growth and substrate degradation rates of CAP. These results indicate that
CAP degrading enzymes are inducible in nature. During incubation with E. fergusonii cells, reduction
intermediates (1-p-nitrophenyl -2-amino-1, 3-propanediol) were observed. The products of CAP
metabolism by species other than E. fergusonii have not yet been characterized. The strains reducing
the antibiotic were chloramphenicol susceptible, indicating that the pathway does not appear to mediate
chloramphenicol resistance. The role of this CAP reduction pathway in the physiology of Klebsiella
pneumonia and E. fergusonii is unknown.
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