纤维蛋白凝胶承载内皮祖细胞移植梗死心肌后细胞自噬变化.doc

 n  纤维蛋白凝胶承载内皮祖细胞移植梗死心 肌后细胞自噬变化 # 张丹，王海杰，伍金红，李志华，谭玉珍** 5 10 （复旦大学上海医学院人体解剖与组织胚胎学系，上海 200032） 摘要：目的 观察心肌梗死后移植内皮祖细胞（endothelial progenitor cells，EPCs）时的细 胞自噬变化，研究自噬维持移植细胞存活和纤维蛋白凝胶保护细胞的作用。方法 通过结扎 左冠状动脉的前降支建立大鼠心肌梗死模型后，在正常区、梗死边缘区和梗死区分别注射从 人脐带血中分选的 EPCs。2 h 后取注射处组织，作半薄切片，观察纤维蛋白凝胶承载的 EPCs 分布。定位后作超薄切片，透射电镜下观察 EPCs 的自噬结构变化以及纤维蛋白凝胶与 EPCs 和心肌的相容性。结果 与正常区相比，梗死边缘区发生自噬的 EPCs 增多，细胞内自噬结 构也增多。梗死区的移植细胞的自噬显著增强，有的细胞坏死或凋亡。纤维蛋白凝胶与移植 细胞和心肌的相容性良好，移植的 EPCs 在纤维蛋白凝胶中充分伸展，有的 EPCs 与心肌细 胞粘附。结论 在梗死边缘区移植 EPCs 后，轻度缺血刺激细胞发生自噬，这有利于维持移 15 20 25 30 35 40 植细胞存活。纤维蛋白凝胶承载 EPCs 可避免细胞丢失和促进存活。 关键词：自噬；内皮祖细胞；细胞移植；纤维蛋白；心肌梗死 中图分类号：Q253 Autophagic changes of progenitor endothelial cells carried with fibrin glue after transplantation into the infracted myocardium ZHANG Dan, WANG Haijie, WU Jinhong, LI Zhihua, TAN Yuzhen (Department of Anatomy, Histology and Embryology, Shanghai Medacal School of Fudan University, Shanghai 200032) Abstract: Objective To investigate autophagic changes of fibrin glue-carried endothelial progenitor cells (EPCs) after transplantation into the infracted myocardium and explore effects of autophagy on maintaining the implanted cells to survive and fibrin on protecting the cells. Methods Rat models of myocardium infarction were established with ligating the anterior descending branch of the left coronary artery. EPCs sorted from human umbilical cord blood were injected into the myocardium at normal region, periphery of infarcted region and infarcted region. After transplantation for two hours, the tissues at injection sites were removed, the semithin sections were prepared. Distribution of EPCs carried with fibrin glue were examined. After positioning the implanted cells, the ultrathin sections were prepared. Changes of autophagic structures in EPCs and compatibility of fibrin with EPCs and myocardium were evaluated. Results Comparing with the normal region, the autophagic EPCs in the periphery of the infarcted region increased, the autophagic structures in the cells also increase